Research / Development / Patent

Research of functional food/functional material

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  「Composition having lipolysis promoting action」Patent overview

  The "composition having a lipolysis-promoting action" of the present invention is one having an excellent lipolysis and blood pressure lowering action due to a synergistic action with "salmon milt extract/beer yeast extract/young barley leaf extract/chicken collagen". is.

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  「Lipolysis promoting action」

  The experiment involves (i) culturing fat precursor cells in mice, adding a fat cell differentiation-inducing agent to induce differentiation to fat cells, (ii) further culturing the cells in an insulin-containing medium, and then, it added “Salmon white fruit extract, beer yeast extract, barley young leaf extract, chicken collagen”. (iii) The fat decomposition effect was measured from the amount of glycerol because the fat decomposes to produce glycerol. The barley young leaf extract had a lipolytic action separately, but the mixture added with each component has a lipolytic action superior to the state of　the barley young leaf extract.
  
  

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  「ACE inhibitory activity」

  As an experiment of the hypotensive action, a substance obtained by adding a digestive enzyme (Pepsin trypsin) to each component and mixture and then freeze-drying the deactivated processing liquid is used to measure the absorbance value. It has been found that the cause of elevated blood pressure is angiotensin I's ACE-converting enzyme, which results in angiotensin II, which is caused by the constriction of blood vessels that angiotensin II has. On the other hand, the enzyme-treated mixture showed good ACE inhibitory activity and expected blood pressure drop.
  
  

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  「Adiponectin production accelerating composition」Patent overview

  As the main component of salmon milt extract, the invented adiponectin production accelerating composition has beer yeast extract, chicken collagen and mineral-containing yeast and excellent adiponectin production accelerating effect by mixing them.
  
  ◎Adiponectin is physiological active substance (cytokine) secreted from fat cells and one of good substance. It repairs damaged blood vessels (standard value 5～10㎍/ml) occurring in considerable quantity in the blood and prevents and improves arteriosclerosis. And insulin will work better so that it will be useful for prevention and improvement of diabetes. The more visceral fat increases, secretion of Adiponectin decreases, blood concentration is reduced.Because adiponectin itself can’t be ingested directly, it is important to ingest nutrients promoting adiponectin secretion.

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  「Adiponectin production accelerating effect」

  “Example”
  We cultivated mouse fibroblast cell, induced and guided fat cells, then enlarged fat cells. After cultivated this cell in insulin-containing　medium24hours and decreased insulin secretion, we added composition of “salmon milt extract/ beer yeast extract/ chicken collagen and mineral-containing
  yeast” as 50㎍/ml and 250㎍/ml. Then we found the result that adiponectin concentration was increased compared with additive‐free (control)condition, after culturing 48 hours at 37℃.
  
  
  

Adiponectin production accelerating composition Patent overview

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  Patent acquisition country

  Japan

  Patent acquisition date

  May 27, 2011

  Patent No
  4750213

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  Patent acquisition country

  The United States of America

  Patent acquisition date

  November 11, 2014

  Patent No
  US8883138B2

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  Patent acquisition country

  People’s Republic of China

  Patent acquisition date

  December 3, 2014

  Patent No
  ZL201180024775.7

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  Patent acquisition country

  Taiwan

  Patent acquisition date

  Maych 1,2015

  Patent No
  I474829

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  Patent acquisition country

  Macao

  Patent acquisition date

  June 17, 2015

  Patent No
  J/001685

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  Patent acquisition country

  Mexico

  Patent acquisition date

  June 22, 2015

  Patent No
  330956

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  Patent acquisition country

  Hong Kong

  Patent acquisition date

  July 24,2015

  Patent No
  HK1176876

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  Patent acquisition country

  Indonesia

  Patent acquisition date

  September 30, 2015

  Patent No
  IDP000039589

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  Patent acquisition country

  Russia

  Patent acquisition date

  October 27, 2015

  Patent No
  2567053

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  Patent acquisition country

  Malaysia

  Patent acquisition date

  March 15, 2017

  Patent No
  MY-160509-A

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  Patent acquisition country

  Canada

  Patent acquisition date

  July 11, 2017

  Patent No
  2798556

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  Patent acquisition country

  Republic of India

  Patent acquisition date

  November 13,2019

  Patent No
  324913

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  Patent acquisition country

  Socialist Republic of Vietnam　

  Patent acquisition date

  March 17,2020

  Patent No
  23477

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  Patent acquisition country

  Federetive Republic of Brazil

  Patent acquisition date

  November 17,2020

Composition having lipolysis-promotive effect Patent overview

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  Patent acquisition country

  Japan

  Patent acquisition date

  April 16, 2010

  Patent No
  4493725

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  Patent acquisition country

  People’s Republic of China

  Patent acquisition date

  December 17, 2014

  Patent No
  ZL201080043430.1

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  Patent acquisition country

  Macao

  Patent acquisition date

  June 17, 2015

  Patent No
  J/001687

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  Patent acquisition country

  Hong Kong

  Patent acquisition date

  September 18, 2015

  Patent No
  HK1169306

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  Patent acquisition country

  Taiwan

  Patent acquisition date

  November 20, 2015

  Patent No
  I520741

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  Patent acquisition country

  The United States of America

  Patent acquisition date

  July 26, 2016

  Patent No
  US9399043B2

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  Patent acquisition country

  Korea

  Patent acquisition date

  July 19, 2017

  Patent No
  10-1761112

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  Patent acquisition country

  Republic of India

  Patent acquisition date

  March 13,2019

  Patent No
  309156

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  Patent acquisition country

  Federative Republic of Brazil

  Patent acquisition date

  June 16,2020

  Patent No
  BR112012006872-5

Research and development of cosmeceuticals.

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  「Applied the patent for ‘Anti-aging for skin and anti-aging-related gene expression regulator’」Patent overview

  We have discovered that the mixture of “special low-molecular weight DNA” and “soybean extracts (F polyamine)” has a synergistic effect in anti-aging of skin and adjusting expression of anti-aging-related genes.

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  「Anti-aging agent for skin」Cell proliferation action

  We have found that the mixture of special low-molecular weight DNA and soybean extracts (F polyamine) has synergistic effects on activating fibroblasts; that is, effects on cell proliferation promoting action, collagen production promoting action, and hyaluronan production promoting action.
  
  〈Test using human-derived dermal fibroblast cell line〉
  Test result (1) Cell proliferation action
  Cell proliferation rate increased by 133% by mixing 0.01㎎/ml of special low-molecular weight DNA and 0.27㎎/ml of soybean extracts (F polyamine).
  Cell proliferation rate increased by 141% by mixing 0.1㎎/ml of special low-molecular weight DNA and 0.27㎎/ml of soybean extracts (F polyamine).
  
  
  

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  「 Anti-aging agent for skin」Collagen production promoting action

  We have found that the mixture of special low-molecular weight DNA and soybean extracts (F polyamine) has synergistic effects on activating fibroblasts; that is, effects on cell proliferation promoting action, collagen production promoting action, and hyaluronan production promoting action.
  
  〈Test using human-derived dermal fibroblast cell line〉
  Test result (2) Collagen production promoting action
  Collagen production promoting rate increased by 178% by mixing 0.01㎎/ml of special low-molecular weight DNA and 0.27㎎/ml of soybean extracts (F polyamine).
  Collagen production promoting rate increased by 231% by mixing 0.1㎎/ml of special low-molecular weight DNA and 0.27㎎/ml of soybean extracts (F polyamine).
  

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  「Anti-aging agent for skin」Hyaluronic acid production promoting action

  We have found that the mixture of special low-molecular weight DNA and soybean extracts (F polyamine) has synergistic effects on activating fibroblasts; that is, effects on cell proliferation promoting action, collagen production promoting action, and hyaluronan production promoting action.
  
  〈Test using human-derived dermal fibroblast cell line〉
  Test result (3) Hyaluronic acid production promoting action
  Hyaluronan production promoting rate increased by 133% by mixing 0.01㎎/ml of special low-molecular weight DNA and 0.27㎎/ml of soybean extracts (F polyamine).
  Hyaluronan production promoting rate increased by 140% by mixing 0.1㎎/ml of special low-molecular weight DNA and 0.27㎎/ml of soybean extracts (F polyamine).
  

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  「Agent for adjusting expression of anti-aging-related genes of skin」Promoting gene expression related to hyaluronan synthesis

  Furthermore, we have also found that the mixture of special low-molecular weight DNA and soybean extracts (F polyamine) facilitates the expression of genes related to hyaluronic acid synthesis, while reducing expression of genes related to hyaluronic acid degradation.
  
  〈Test using human-derived dermal fibroblast cell line〉
  Test result (1) Expression analysis of hyaluronan synthase-1 (HAS1) genes
  Hyaluronic acid synthesis enzyme-1 genes (HAS1) increased by 1.51±0.26 by mixing 0.1㎎/ml of special low-molecular weight DNA and 0.27㎎/ml of soybean extracts (F polyamine).
  

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  「Agent for adjusting expression of anti-aging-related genes of skin」Reduced gene expression for hyaluronan degradation

  Furthermore, we have also found that the mixture of special low-molecular weight DNA and soybean extracts (F polyamine) facilitates the expression of genes related to hyaluronic acid synthesis, while reducing expression of genes related to hyaluronic acid degradation.
  
  〈Test using human-derived dermal fibroblast cell line〉
  Test result (2) Expression analysis of genes of hyaluronan-degrading enzyme (HYAL1)
  Hyaluronan-degrading enzyme - I genes (HYAL1) decreased by 0.58±0.11 by mixing 0.01㎎/ml of special low-molecular weight DNA and 0.27㎎/ml of soybean extracts (F polyamine).

Applied the patent for ‘The anti-aging drug for the skin or the gene expression regulation drug’ Patent overview

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  Patent acquisition country

  Japan

  Patent acquisition date

  July 19,2019

  Patent No
  6555674

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  Patent acquisition country

  People’s Republic of China

  Patent acquisition date

  March 26,2021

  Patent No
  ZL201980001839.8

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  Patent acquisition country

  Russia

  Patent acquisition date

  April 3,2021

  Patent No
  2744251

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  Patent acquisition country

  Hong Kong

  Patent acquisition date

  JULY 23,2021

  Patent No
  HK40012865

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  Patent acquisition country

  Macau

  Patent acquisition date

  September 1, 2021

  Patent No
  J/004964

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  Patent acquisition country

  India

  Patent acquisition date

  November 12, 2021

  Patent No
  381670

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  Patent acquisition country

  Taiwan

  Patent acquisition date

  November 12, 2021

  Patent No
  1747001

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  Patent acquisition country

  Republic of Korea

  Patent acquisition date

  February 18,2022

  Patent No
  10-2366340

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  Patent acquisition country

  Malaysia

  Patent acquisition date

  December 15, 2022

  Patent No
  MY-194768-A

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  Patent acquisition country

  United States of America

  Patent acquisition date

  January 17, 2023

  Patent No
  US 11,554,092 B2

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  Patent acquisition country

  Mexico

  Patent acquisition date

  April 18, 2023

  Patent No
  401869